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BD FACSDiva™ Software v8.0 Administrative Tasks BD Biosciences bdbiosciences.com Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon Brilliant Violet 421, BD Horizon V450 450/40 656738 na na BD Horizon Brilliant Violet 510, BD Horizon V500 525/50 656739 505LP 656745 BD Horizon Brilliant™ Violet 605 610/20 656740 595LP 656746 BD Horizon Brilliant™ Violet 650 660/20 656741 630LP 656747 BD Horizon™ BUV737 740/35 690LP 659997 BD Horizon™ BUV805 820/60 770LP 659998 Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon™ BV421 450/40 656738 – – BD Horizon™ BV510 525/50 656739 505LP 656745 BD Horizon™ BV605 610/20 656740 595LP 656746 BD Horizon™ BV650 660/20 656741 630LP 656747 BD Horizon™ BV711 710/50 656742 Flow Cytometry Service BD LSR Fortessa SORP – How to Use Guide 5 4 Create a New Specimen (click the icon with a syringe). 5 Select Tube_001 (inside the syringe) by clicking in the green arrow. 6 Do 3 PRIMEs with the SIP 2 arm open and no tube installed. Then set the cytometer to RUN. 7 Acquire and record dH 2O for 3 minutes in HIGH (name it ‘Initial’).

Bd fortessa filters

DETECTOR Ghost Dye Red 780. 633. 780. Tombo. 750. 780/60.

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Proliferation BD Fortessa Configuration. KCRB.

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Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3. Depressurize the sheath container by pulling up on the vent valve. 4.

With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with BD Horizon Brilliant ™ dyes, so you can focus on what matters most—your research. 4) Start BD FACSDiva software. Group User and Password 5) If working with bacteria, open the black nob in front of the FSC. 6) Open the optical filter compartment (on the left side of the Fortessa and two small ports in front) to make sure that the filters necessary for detecting your fluorophores are in place. BD Fortessa Laser Detector name (band pass filter) *Suggested Fluorochromes Blue (488nm) FSC SSC 488 (530/30) Fitc, Alexa488, GFP, YFP 488 (695/40) PerCPCy5.5 BD FACSArray™ bioanalyzer Green Diode 532 Yellow PE Far Red PerCP-Cy5.5 PE-Cy7 Red Diode 635 Red APC Alexa Fluor® 647 Infrared BD APC-H7 APC-Cy7 *BD FACSCalibur™ flow cytometry system Argon 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE FL3 Red PE-Cy5a PerCP PerCP-Cy5.5 PE-Cy7 Red Diode 635 FL4 Red APCa Alexa Fluor® 647 *BD FACSCanto™ 1.844.8.BD.LIFE (844.823.5433) Now with one customer support number, it's easier for you to review your order status, place a new order, get product information or learn about product availability. BD support quick links filter Side Scatter Detector Photomultiplier tube (PMT) with a 488/10 BP filter Emission Optical Design Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays.
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FITC/BB5815/VioBright FITC/Alexa488. 695/40. PerCP*, PerCP-Cy5.5™, PE-Cy5™ (PC5), PE-Cy5.5™**, PerCP-eFluor® 710, The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry simultaneously with a defined set of optical filters that meet or exceed the The cytometer configuration defines which filter and mirror are installed at each detector. BD FACSDiva software provides a BD base configuration for your BD The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed BD LSR-Fortessa Optical Configuration · BD LSRII-1 Default Filter Configuration The BD LSRFortessa optical layout allows for up to four lasers. Laser power Photomultiplier tube (PMT) with a 488/10 bandpass filter.

Guide for LSR Fortessa. Laser Detector. Filter ( nm). Example Fluorophores.
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4) Start BD FACSDiva software. Group User and Password 5) If working with bacteria, open the black nob in front of the FSC. 6) Open the optical filter compartment (on the left side of the Fortessa and two small ports in front) to make sure that the filters necessary for detecting your fluorophores are in place.

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Confirm the computer was OFF before you do this. Otherwise, switch OFF the computer first. Be aware that the lasers take about 15 minutes to warm up and BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3.